This is something we do all the time in StatQuest. It’s useful for exploring statistics (which is fun!). So learn about it if you don’t already know…
StatQuest: One vs Two-Tailed P-values
Here’s the deal!
StatQuest: edgeR part 1, Library Normalization
Here’s the dope on how edgeR normalized libraries…
StatQuest: DESeq2 part 1: Library Normalization
DESeq2 and edgeR are complicated programs for identifying differential gene expression from high-throughput sequencing data. This is the first in a long series of videos that explains how these programs work.